Single-cell study of the extracellular matrix effect on cell growth by in situ imaging of gene expression† †Electronic supplementary information (ESI) available: Sequence information, in vitro RCA and RT-qPCR data. See DOI: 10.1039/c7sc03880a

Simultaneous visualization of the subfemtomolar expression of microRNA and microRNA target gene using HILO microscopy† †Electronic supplementary information (ESI) available: The LED device for the sample photobleaching, a schematic presentation of HILO microscopy, fluorescence spectra and hybridization curves of the molecular beacons, the linear correlation between the miRNA fluorescence intensity and the miRNA copy number, a validation of the miRNA adsorption and miRNA target gene expression via RT-qPCR, a validation of RT-qPCR using capillary electrophoresis, the reproducibility of RT-qPCR and Poisson distribution of the miRNA pipetting as well as a complete list of the oligonucleotides used in this study. See DOI: 10.1039/c7sc02701j Click here for additional data file.

Department of Chemistry, Tunghai Univ [email protected] Graduate Institute of Oncology, College o Taipei 100, Taiwan Department of Internal Medicine, National Taiwan Department of Oncology, National Taiwan † Electronic supplementary information sample photobleaching, a schematic uorescence spectra and hybridization linear correlation between the miRNA  copy number, a validation of the miRN ex...

EVALUATION OF THE EFFECTS OF IBUPROFEN CYTOTOXIC DOSE ON EXPRESSION LEVEL OF EXTRACELLULAR MATRIX DEGRADING MMP-9 AND ANTI-METASTASIS NM23 GENES IN CERVICAL CANCER CELLS

Background & Aim: Although studies have shown that ibuprofen has anticancer effects on many cancer cells, the mechanism of the ibuprofen anticancer effect in cancer cells is not still well understood. The aim of this study was to investigate the effect of cytotoxic concentration of ibuprofen on the expression level of MMP-9 and NM23 genes in cervical cancer cells. Materials & Methods: During t...

Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining† †Electronic supplementary information (ESI) available: Brightfield and fluorescence cell imaging, excitation and emission spectra. See DOI: 10.1039/c7sc01872j Click here for additional data file.

Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography† †Electronic supplementary information (ESI) available: Experimental procedures for microarray fabrication, deprotection and cleavage as well as LC-MS conditions and spectra of all array eluates. See DOI: 10.1039/c4cc05771f Click here for additional data file.

Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using ligh...

In situ characterization of advanced glycation end products (AGEs) in collagen and model extracellular matrix by solid state NMR† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7cc06624d

Non-enzymatic glycation of extracellular matrix with (U-13C5)-d-ribose-5-phosphate (R5P), enables in situ 2D ssNMR identification of many deleterious protein modifications and crosslinks, including previously unreported oxalamido and hemiaminal (CH3-CH(OH)NHR) substructures. Changes in charged residue proportions and distribution may be as important as crosslinking in provoking and understandin...